5MO3
Crystal structure of DC8E8 Fab in the complex with a 14-mer tau peptide at pH 8.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-05 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9999 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.728, 60.128, 69.315 |
Unit cell angles | 90.00, 109.25, 90.00 |
Refinement procedure
Resolution | 65.440 - 1.690 |
R-factor | 0.1917 |
Rwork | 0.189 |
R-free | 0.23470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4OZ4 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.580 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 65.440 | 65.440 | 1.730 |
High resolution limit [Å] | 1.690 | 7.560 | 1.690 |
Rmerge | 0.059 | 0.021 | 1.379 |
Rmeas | 0.071 | ||
Total number of observations | 129645 | ||
Number of reflections | 41553 | ||
<I/σ(I)> | 13.83 | 50.1 | 0.89 |
Completeness [%] | 81.1 | 95 | 51.3 |
Redundancy | 3.12 | 2.827 | 3.095 |
CC(1/2) | 0.999 | 0.998 | 0.448 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 30% PEG 4000, 0.1 M Tris, 0.2 M LiSO4 |