5MO3

Crystal structure of DC8E8 Fab in the complex with a 14-mer tau peptide at pH 8.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-05
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9999
Spacegroup name	P 1 21 1
Unit cell lengths	58.728, 60.128, 69.315
Unit cell angles	90.00, 109.25, 90.00

Refinement procedure

Resolution	65.440 - 1.690
R-factor	0.1917
Rwork	0.189
R-free	0.23470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4OZ4
RMSD bond length	0.011
RMSD bond angle	1.580
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.440	65.440	1.730
High resolution limit [Å]	1.690	7.560	1.690
Rmerge	0.059	0.021	1.379
Rmeas	0.071
Total number of observations	129645
Number of reflections	41553
<I/σ(I)>	13.83	50.1	0.89
Completeness [%]	81.1	95	51.3
Redundancy	3.12	2.827	3.095
CC(1/2)	0.999	0.998	0.448

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	30% PEG 4000, 0.1 M Tris, 0.2 M LiSO4