5MN2

Cocrystal structure of Fc gamma receptor IIIa interacting with Affimer G3, a specific binding protein which blocks IgG binding to the receptor.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-08-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 1 21 1
Unit cell lengths	64.985, 59.925, 100.001
Unit cell angles	90.00, 102.09, 90.00

Refinement procedure

Resolution	43.390 - 2.350
R-factor	0.2075
Rwork	0.205
R-free	0.24430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	A subsection of a complex of an Adhiron bound to a soluble protein (manuscript in preparation) was used as a search model.
RMSD bond length	0.002
RMSD bond angle	0.459
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.100	2.450
High resolution limit [Å]	2.350	2.350
Rmerge	0.040	0.807
Number of reflections	30227
<I/σ(I)>	15.7	1.2
Completeness [%]	95.8	97.2
Redundancy	2.7	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	20.0% v/v Glycerol 16.0% w/v Polyethylene glycol 8000 0.04 M Potassium di-Hydrogen Phosphate