5MMA
Crystal structure of the Prototype Foamy Virus (PFV) intasome in complex with magnesium and the INSTI XZ379 (compound 5'g)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-19 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97957 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 159.570, 159.570, 124.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.360 - 2.550 |
R-factor | 0.1801 |
Rwork | 0.179 |
R-free | 0.20730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4be2 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.563 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.360 | 2.620 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.062 | 1.490 |
Number of reflections | 52567 | |
<I/σ(I)> | 26.6 | 2 |
Completeness [%] | 99.8 | 98.8 |
Redundancy | 11.5 | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.35 M ammonium sulfate, 25% (v/v) glycerol, 4.8% (v/v) 1,6-hexanediol, 50 mM Mes-NaOH, 1mM EDTA, pH 6.5 |