5MM1

Dolichyl phosphate mannose synthase in complex with GDP and dolichyl phosphate mannose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	C 2 2 21
Unit cell lengths	90.110, 144.540, 96.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.390 - 2.600
R-factor	0.244
Rwork	0.238
R-free	0.30300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mlz
RMSD bond length	0.008
RMSD bond angle	1.217
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.385	2.700
High resolution limit [Å]	2.600	2.600
Number of reflections	19735
<I/σ(I)>	14	1.1
Completeness [%]	99.6	99.4
Redundancy	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	0.2 M potassium chloride, 0.1 M trisodium citrate (pH 5.5), 37% (v/v) pentaerythritol propoxylate (5/4 PO/OH), 50 mM Hepes (pH 7.5), 150 mM NaCl, 10% (v/v) glycerol, 0.05% LDAO, 5 mM GDP-mannose, 5 mM MgCl2, 0.25 mM C55 dolichyl phosphate