5MLZ
Dolichyl phosphate mannose synthase in complex with GDP and Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97857 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 90.870, 146.210, 95.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.016 - 2.000 |
R-factor | 0.2418 |
Rwork | 0.241 |
R-free | 0.25710 |
Structure solution method | MIR |
RMSD bond length | 0.005 |
RMSD bond angle | 1.051 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | autoSHARP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.950 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 43116 | |
<I/σ(I)> | 29.2 | 2.2 |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 13.3 | |
CC(1/2) | 0.876 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 0.2 M potassium chloride, 0.1 M trisodium citrate (pH 5.5), 37% (v/v) pentaerythritol propoxylate (5/4 PO/OH), 50 mM Hepes (pH 7.5), 150 mM NaCl, 10% (v/v) glycerol, 0.05% LDAO, 5 mM GDP, 5 mM MgCl2 |