5MKI

Crystal structure of SmAP (LSm) protein from Methanococcus vannielii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.918400
Spacegroup name	P 1 21 1
Unit cell lengths	69.671, 114.221, 71.993
Unit cell angles	90.00, 113.70, 90.00

Refinement procedure

Resolution	43.164 - 2.048
R-factor	0.1939
Rwork	0.191
R-free	0.25660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i4k
RMSD bond length	0.007
RMSD bond angle	0.880
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.048	2.048
Rmerge	0.083	0.800
Number of reflections	63608
<I/σ(I)>	11.77	1.5
Completeness [%]	97.9	95.38
Redundancy	3.8	3.7
CC(1/2)	0.998	0.638

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	Protein 10 mg/ml, 30% PEG400, 0,1M HEPES, pH 7.5, 0,2 M CaCl2