5MJJ
Single-shot pink beam serial crystallography: Lysozyme
Experimental procedure
Experimental method | LAUE |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-D |
Synchrotron site | APS |
Beamline | 14-BM-D |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2016-07-10 |
Detector | RAYONIX MX340-HS |
Wavelength(s) | 1.1-1.3 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.100, 79.100, 37.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.014 - 1.750 |
R-factor | 0.163947591569 |
Rwork | 0.161 |
R-free | 0.18829 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fek |
RMSD bond length | 0.003 |
RMSD bond angle | 0.473 |
Data reduction software | Precognition/Epinorm (RenzResearch) |
Phasing software | PHENIX (1.10.1-2155_9999) |
Refinement software | PHENIX (1.10.1-2155_9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.830 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.076 | |
Number of reflections | 9538 | |
<I/σ(I)> | 28.5 | |
Completeness [%] | 75.5 | 25.21 |
Redundancy | 9.2 | 15.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 277 | 1M NaCl, 35% (v/v) ethylene glycol, 20% (w/v) PEG4000, 50 mM sodium acetate pH 3.5 |