5MIW
X-ray structure uridine phosphorylase from Vibrio cholerae in complex with uracil at 1.28 A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.3 |
| Synchrotron site | BESSY |
| Beamline | 14.3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-23 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.896 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.513, 71.948, 89.332 |
| Unit cell angles | 69.22, 72.20, 85.60 |
Refinement procedure
| Resolution | 32.030 - 1.280 |
| R-factor | 0.133 |
| Rwork | 0.132 |
| R-free | 0.16900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4oeh |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.030 | 1.360 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.113 | 0.888 |
| Rmeas | 0.131 | 0.035 |
| Total number of observations | 1344228 | |
| Number of reflections | 345622 | 13600 |
| <I/σ(I)> | 10.5 | 1.79 |
| Completeness [%] | 94.0 | 82.9 |
| Redundancy | 3.45 | 3.826 |
| CC(1/2) | 0.997 | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG4000, 0.1M CHES, 0.2M MgCl2x6H2O |
