5MIU
G307E variant of Murine Apoptosis Inducing Factor (oxidized state)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-05 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97895 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.040, 110.060, 119.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.700 - 3.500 |
| R-factor | 0.21524 |
| Rwork | 0.212 |
| R-free | 0.27857 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gd3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.302 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.700 | 3.590 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmeas | 0.039 | 0.130 |
| Number of reflections | 16448 | |
| <I/σ(I)> | 5.3 | 1.8 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 5.4 | 5.7 |
| CC(1/2) | 0.973 | 0.735 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG 4000,0.2 M sodium acetate, pH 8.5 |
