5MHK

ICP4 DNA-binding domain in complex with 19mer DNA duplex from its own promoter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-03
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	61.510, 100.730, 201.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.300 - 2.280
R-factor	0.2131
Rwork	0.211
R-free	0.24290
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.559
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.300	2.362
High resolution limit [Å]	2.280	2.280
Rmerge	0.161	1.333
Number of reflections	58110
<I/σ(I)>	8.69	1.39
Completeness [%]	99.9	99.95
Redundancy	6.5	6.5
CC(1/2)		0.574

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	0.2M Ammonium sulfate, 0.1 Bis/Tris pH 5.5 & 25% w/v PEG3350 [SG1 B8 Molecular Dimensions]. Cryoprotected with 20% PEG 200