5MGV
Kinetic and Structural Changes in HsmtPheRS, Induced by Pathogenic Mutations in Human FARS2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 295 |
Detector technology | PIXEL |
Collection date | 2016-02-19 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.770, 90.370, 95.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.050 |
Rwork | 0.228 |
R-free | 0.27110 |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.050 |
High resolution limit [Å] | 2.050 |
Number of reflections | 28291 |
<I/σ(I)> | 25.22 |
Completeness [%] | 100.0 |
Redundancy | 19.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 2 mM DTT, 100 mM bis-Tris propane pH 7 and 1.8 M sodium acetate pH 7 |