5MGU

Kinetic and Structural Changes in HsmtPheRS, Induced by Pathogenic Mutations in Human FARS2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	295
Detector technology	PIXEL
Collection date	2016-02-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	54.539, 90.133, 97.467
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.662 - 1.890
R-factor	0.2039
Rwork	0.202
R-free	0.24380
RMSD bond length	0.007
RMSD bond angle	0.971
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	46.662
High resolution limit [Å]	1.890
Number of reflections	38365
<I/σ(I)>	22.14
Completeness [%]	98.0
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	2 mM DTT, 100 mM bis-Tris propane pH 7 and 1.8 M sodium acetate pH 7