5MGB
Crystal Structure of Rat Peroxisomal Multifunctional enzyme Type-1 (RPMFE1) Complexed with Acetoacetyl-CoA and NAD
Replaces: 5AAKExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.230, 125.820, 223.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.560 - 2.800 |
R-factor | 0.21176 |
Rwork | 0.209 |
R-free | 0.26104 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2x58 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.311 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC (5.8.0155) |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.560 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.130 | 0.380 |
Number of reflections | 38432 | |
<I/σ(I)> | 8 | 2.4 |
Completeness [%] | 83.8 | 84 |
Redundancy | 3.8 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 295 | 100 mM MES pH 6.0, 150 mM Ammonium sulphate, 15 % w/v PEG4000 |