5MFE
Designed armadillo repeat protein YIIIM5AII in complex with (RR)4 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-06 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.000, 103.673, 134.182 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.350 - 1.950 |
R-factor | 0.183 |
Rwork | 0.181 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5mfc |
RMSD bond length | 0.010 |
RMSD bond angle | 1.130 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.350 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.241 | 3.680 |
Number of reflections | 83955 | |
<I/σ(I)> | 7.94 | 0.6 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 8.6 | 8.8 |
CC(1/2) | 0.155 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | PEG8000 5%, MPD 27.27%, NaCacodylate 0.1M pH7 200mM CaCl2 |