5MEF
Cyanothece lipoxygenase 2 (CspLOX2) variant - L304F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.400, 166.260, 166.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.204 - 2.357 |
| R-factor | 0.192 |
| Rwork | 0.191 |
| R-free | 0.21620 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5med |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.707 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.6) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.210 | 2.460 | |
| High resolution limit [Å] | 2.357 | 50.000 | 2.357 |
| Rmerge | 0.041 | 0.533 | |
| Number of reflections | 62963 | ||
| <I/σ(I)> | 18.51 | 2.7 | |
| Completeness [%] | 99.2 | 99.5 | |
| Redundancy | 3.8 | ||
| CC(1/2) | 0.999 | 0.807 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 15% PEG 4000, 12% glycerol, 0.1 M MES/imidazole pH 6.1 |
