5MCV
New Insights into the Role of DNA Shape on Its Recognition by p53 Proteins (complex p53DBD-LWC1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.88560 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 138.078, 49.533, 68.047 |
Unit cell angles | 90.00, 92.91, 90.00 |
Refinement procedure
Resolution | 34.475 - 1.600 |
R-factor | 0.1558 |
Rwork | 0.154 |
R-free | 0.18780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDBID 1TSR |
RMSD bond length | 0.006 |
RMSD bond angle | 0.968 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (2.5.7) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.000 | 35.000 | 1.630 |
High resolution limit [Å] | 1.600 | 4.340 | 1.600 |
Rmerge | 0.073 | 0.045 | 0.394 |
Rmeas | 0.080 | 0.049 | 0.448 |
Rpim | 0.033 | 0.020 | 0.209 |
Total number of observations | 346296 | ||
Number of reflections | 60494 | ||
<I/σ(I)> | 5.2 | ||
Completeness [%] | 99.4 | 99.5 | 97.5 |
Redundancy | 5.7 | 5.9 | 4.3 |
CC(1/2) | 0.999 | 0.940 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8.8 | 292 | 0.02 M Citric acid, 0.08 M BIS-TRIS propane, 16% w/v Polyethylene glycol 3,350 |