5MAC

Crystal structure of decameric Methanococcoides burtonii Rubisco complexed with 2-carboxyarabinitol bisphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 3 2 1
Unit cell lengths	273.760, 273.760, 96.740
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.370 - 2.600
R-factor	0.191
Rwork	0.189
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lf1
RMSD bond length	0.010
RMSD bond angle	1.200
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.370	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	126745
<I/σ(I)>	10.8	1.3
Completeness [%]	99.7	93.7
Redundancy	10.2	8.5
CC(1/2)	0.994	0.231

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	PEG 20K, polyglutamic acid, potassium bromide, magnesium chloride, sodium bicarbonate, ethylenediaminetetraacetic acid, Tris, HEPES