5MAC
Crystal structure of decameric Methanococcoides burtonii Rubisco complexed with 2-carboxyarabinitol bisphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 273.760, 273.760, 96.740 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.370 - 2.600 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lf1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.370 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 126745 | |
| <I/σ(I)> | 10.8 | 1.3 |
| Completeness [%] | 99.7 | 93.7 |
| Redundancy | 10.2 | 8.5 |
| CC(1/2) | 0.994 | 0.231 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | PEG 20K, polyglutamic acid, potassium bromide, magnesium chloride, sodium bicarbonate, ethylenediaminetetraacetic acid, Tris, HEPES |
