5M8I

Solution structure of CUG-BP2 RRM3 in complex with 5'-UUUAA-3' RNA

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	AVANCE III		600
2	Bruker	AVANCE III HD		600
3	Bruker	AVANCE III		900
4	Bruker	AVANCE III		750
5	Bruker	AVANCE III		700

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	2D 1H-15N HSQC
10	1	2	2D 1H-1H NOESY
11	1	2	2D F2 13C-filtered 1H-1H NOESY
12	1	3	natural abundance 2D 13C-1H HSQC
13	1	2	3D F1 13C-filtered 1H-1H NOESY 13C-1H HSQC aliphatic
14	1	2	3D F1 13C-filtered 1H-1H NOESY 13C-1H HSQC aromatic
2	1	1	2D 1H-13C HSQC aliphatic
3	1	1	2D 1H-13C HSQC aromatic
4	1	1	3D 1H-15N NOESY
5	1	1	3D 1H-13C NOESY aliphatic
6	1	1	3D 1H-13C NOESY aromatic
7	1	1	2D 1H-15N HSQC long range
8	1	1	3D HNHA
9	1	2	2D 1H-1H TOCSY

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	5.8	ambient	298

Conformers

Conformers Calculated Total Number	50
Conformers Submitted Total Number	20