5M7V

Translation initiation factor 4E in complex with (RP)-m2(7,2'O)GppSpG mRNA 5' cap analog (beta-S-ARCA D1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918
Spacegroup name	P 1
Unit cell lengths	38.049, 38.057, 146.942
Unit cell angles	88.60, 95.40, 103.61

Refinement procedure

Resolution	36.988 - 1.740
R-factor	0.2376
Rwork	0.236
R-free	0.28760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l8b
RMSD bond length	0.007
RMSD bond angle	0.975
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.840
High resolution limit [Å]	1.740	1.740
Rmerge	0.054	0.347
Number of reflections	77683
<I/σ(I)>	9.68	2
Completeness [%]	94.6	93.6
Redundancy	1.776	1.724
CC(1/2)	0.997	0.716

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	18% Jeffamine, 0.1M HEPES pH 7.0