5M7F
Human porphobilinogen deaminase in complex with DPM cofactor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.972422 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.420, 80.950, 76.220 |
Unit cell angles | 90.00, 95.70, 90.00 |
Refinement procedure
Resolution | 29.770 - 2.780 |
R-factor | 0.222 |
Rwork | 0.220 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ecr |
RMSD bond length | 0.010 |
RMSD bond angle | 1.160 |
Data reduction software | XDS (VERSION May 1, 2016) |
Data scaling software | Aimless (version 0.5.23) |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.950 | 2.830 |
High resolution limit [Å] | 2.780 | 2.780 |
Rmerge | 0.247 | 1.265 |
Number of reflections | 21353 | |
<I/σ(I)> | 3.1 | 0.9 |
Completeness [%] | 98.9 | 83.1 |
Redundancy | 3.3 | 2.6 |
CC(1/2) | 0.946 | 0.477 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.1 M sodium cacodylate pH 6.5, 0.2 M sodium chloride, 2 M ammonium sulphate |