5M54
Mechanism of microtubule minus-end recognition and protection by CAMSAP proteins
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | DIRECT ELECTRON DE-20 (5k x 3k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 8 |
| Software | SPIDER,FREALIGN |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
