5M52
Crystal structure of yeast Brr2 full-lenght in complex with Prp8 Jab1 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 179.335, 181.158, 210.348 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.720 - 3.400 |
R-factor | 0.25301 |
Rwork | 0.252 |
R-free | 0.29629 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dca |
RMSD bond length | 0.007 |
RMSD bond angle | 0.937 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.600 |
High resolution limit [Å] | 3.400 | 3.400 |
Number of reflections | 94451 | |
<I/σ(I)> | 7.1 | |
Completeness [%] | 99.6 | |
Redundancy | 6.75 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M Tris-HCl pH 7.5, 10.5 % (w/v) PEG 3350, 0.2 M MgCl |