5M4L
Crystal Structure of Wild-Type Human Prolidase with Mg ions and LeuPro ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 103.605, 106.839, 216.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.750 - 1.490 |
| R-factor | 0.146 |
| Rwork | 0.146 |
| R-free | 0.17100 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.222 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.925 | 1.580 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.069 | 1.177 |
| Number of reflections | 193948 | |
| <I/σ(I)> | 18.29 | 1.6 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 5.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 10mM NaBorate, 690-760mM NaCitrate |
