5M47
Crystal structure of DapF from Corynebacterium glutamicum in complex with D,L-diaminopimelate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-15 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 155.695, 155.695, 155.695 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 110.090 - 2.590 |
| R-factor | 0.1932 |
| Rwork | 0.191 |
| R-free | 0.22920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h2y |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.103 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 110.100 | 2.640 |
| High resolution limit [Å] | 2.590 | 2.600 |
| Rmerge | 0.115 | 0.344 |
| Number of reflections | 19071 | |
| <I/σ(I)> | 21.1 | 4 |
| Completeness [%] | 97.4 | 93.4 |
| Redundancy | 12 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | Sodium citrate, CHES |
