5M34
Structure of cobinamide-bound BtuF mutant W66Y, the periplasmic vitamin B12 binding protein in E.coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 123 |
Detector technology | PIXEL |
Collection date | 2014-12-13 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97794 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 139.830, 90.800, 51.070 |
Unit cell angles | 90.00, 111.25, 90.00 |
Refinement procedure
Resolution | 29.480 - 1.600 |
R-factor | 0.19327 |
Rwork | 0.191 |
R-free | 0.23334 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5m29 |
RMSD bond length | 0.024 |
RMSD bond angle | 2.609 |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.10.1-2155) |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.480 |
High resolution limit [Å] | 1.600 |
Number of reflections | 73585 |
<I/σ(I)> | 9.77 |
Completeness [%] | 93.2 |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | PEG3350 HEPES pH 7 Tryptone |