5M2Z
Crystal structure of the full-length Zika virus NS5 protein (Human isolate Z1106033)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97928 |
| Spacegroup name | P 65 |
| Unit cell lengths | 234.578, 234.578, 406.123 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.323 - 4.800 |
| R-factor | 0.2419 |
| Rwork | 0.241 |
| R-free | 0.26570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5m2x |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.530 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.070 | 5.060 |
| High resolution limit [Å] | 4.800 | 4.800 |
| Rmerge | 0.159 | 1.495 |
| Number of reflections | 61390 | |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 100 mM Sodium Acetate, 1 M di-potassium hydrogen phosphate, 0.95 M sodium di-hydrogen phosphate. |
