5LZP
Binding of the C-terminal GQYL motif of the bacterial proteasome activator Bpa to the 20S proteasome
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK I |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.5 |
Software | EMAN,RELION,CTFFIND,UCSF Chimera,PHENIX |
Symmetry | C7 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |