5LYJ
Tubulin-Combretastatin A4 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-11 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.000030 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.592, 157.170, 180.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.432 - 2.397 |
R-factor | 0.1924 |
Rwork | 0.191 |
R-free | 0.21680 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4i4t |
RMSD bond length | 0.005 |
RMSD bond angle | 0.837 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.432 | 2.560 |
High resolution limit [Å] | 2.397 | 2.397 |
Rmerge | 0.148 | 2.350 |
Number of reflections | 224177 | |
<I/σ(I)> | 13.5 | 1.2 |
Completeness [%] | 99.7 | 99.4 |
Redundancy | 13.6 | 14.1 |
CC(1/2) | 0.999 | 0.456 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.7 | 293 | 6% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 100 mM MES/Imidazole |