5LYJ

Tubulin-Combretastatin A4 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-11
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.000030
Spacegroup name	P 21 21 21
Unit cell lengths	104.592, 157.170, 180.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.432 - 2.397
R-factor	0.1924
Rwork	0.191
R-free	0.21680
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4i4t
RMSD bond length	0.005
RMSD bond angle	0.837
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.432	2.560
High resolution limit [Å]	2.397	2.397
Rmerge	0.148	2.350
Number of reflections	224177
<I/σ(I)>	13.5	1.2
Completeness [%]	99.7	99.4
Redundancy	13.6	14.1
CC(1/2)	0.999	0.456

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.7	293	6% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 100 mM MES/Imidazole