5LYC
Cytochrome c in complex with phosphonato-calix[6]arene
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 54.570, 54.570, 208.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.780 - 1.800 |
| R-factor | 0.1701 |
| Rwork | 0.169 |
| R-free | 0.19940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.796 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.340 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.120 | 1.900 |
| Number of reflections | 30379 | |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 9.1 | 8.7 |
| CC(1/2) | 0.998 | 0.459 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 3.15M ammonium sulfate 100 mM sodium citrate pH 5.0 |
