5LXG
Revised crystal structure of the human adiponectin receptor 1 in an open conformation
Replaces: 3WXVExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-23 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 92.310, 194.110, 74.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.660 - 2.730 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3WXV |
RMSD bond length | 0.008 |
RMSD bond angle | 0.970 |
Data reduction software | HKL-2000 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 97.060 |
High resolution limit [Å] | 2.730 |
Rmerge | 0.171 |
Number of reflections | 17815 |
<I/σ(I)> | 7.934 |
Completeness [%] | 99.7 |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 8 | 293 | 100mM Bicine, 100mM MgCl2, 30% PEG400, pH 8.0, Lipidic mesophase method, temperature 293K |