5LXD
Crystal structure of DYRK2 in complex with EHT 1610 (compound 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-19 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9200 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.240, 130.000, 136.330 |
| Unit cell angles | 90.00, 90.40, 90.00 |
Refinement procedure
| Resolution | 47.040 - 2.580 |
| R-factor | 0.19982 |
| Rwork | 0.197 |
| R-free | 0.25303 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.179 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.040 | 2.720 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.091 | 0.649 |
| Number of reflections | 35477 | |
| <I/σ(I)> | 9.1 | 2 |
| Completeness [%] | 97.8 | 99.4 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 1.5 M Li2SO4 and 0.1 M HEPES, pH 7.5 |
