5LX9

CRYSTAL STRUCTURE OF HUMAN ADIPONECTIN RECEPTOR 2 IN COMPLEX WITH A C18 FREE FATTY ACID AT 2.4 ANGSTROM RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.976251
Spacegroup name	P 21 2 21
Unit cell lengths	74.578, 101.128, 111.577
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	101.130 - 2.400
R-factor	0.183
Rwork	0.181
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.050
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	101.130	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.527	5.681
Number of reflections	33584
<I/σ(I)>	5.5	1.1
Completeness [%]	100.0	99.7
Redundancy	10.5	10.2
CC(1/2)	0.988	0.489

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	30-45% PEG 400, 0.1 M HEPES pH 7.0, and 50-100 mM potassium citrate, 0.01 mM AdipoRon