5LX3
CRYSTAL STRUCTURE OF VISFATIN IN COMPLEX WITH SAR154782.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.510, 107.130, 83.590 |
| Unit cell angles | 90.00, 96.69, 90.00 |
Refinement procedure
| Resolution | 46.930 - 2.100 |
| Rwork | 0.172 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUSTER-TNT |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.930 | 2.230 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.139 | 0.385 |
| Number of reflections | 62781 | |
| <I/σ(I)> | 11.3 | 3 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | Tris 100mM - PEG8000 16% - pH8,5 |
