5LWO
Structure of Spin-labelled T4 lysozyme mutant L115C-R119C-R1 at 100K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.3 |
Synchrotron site | BESSY |
Beamline | 14.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.8946 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.170, 60.170, 97.720 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.299 - 1.183 |
R-factor | 0.1412 |
Rwork | 0.141 |
R-free | 0.16370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jdt |
RMSD bond length | 0.012 |
RMSD bond angle | 1.285 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.250 |
High resolution limit [Å] | 1.180 | 1.180 |
Rmerge | 0.084 | 0.744 |
Number of reflections | 66752 | |
<I/σ(I)> | 10.1 | 1.8 |
Completeness [%] | 98.9 | 96.8 |
Redundancy | 4.4 | 3.9 |
CC(1/2) | 0.998 | 0.772 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 2.0 M NA/K PHOSPHATE, 240 mM NACL, 40 mM 2-HYDROXYETHYL DISULFIDE, PH 6.8 |