5LWF
Structure of a single domain camelid antibody fragment cAb-G10S in complex with the BlaP beta-lactamase from Bacillus licheniformis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-26 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97857 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.278, 121.691, 137.029 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.500 - 2.560 |
R-factor | 0.217 |
Rwork | 0.215 |
R-free | 0.24200 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.010 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.459 |
High resolution limit [Å] | 2.500 |
Number of reflections | 54511 |
<I/σ(I)> | 9.72 |
Completeness [%] | 98.9 |
Redundancy | 3.56 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Mg Acetate, 0.1 M NaCacodylate pH 6.5, 20% PEG8000 |