5LWB

Crystal structure of human JARID1B in complex with S40650a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97949
Spacegroup name	P 65 2 2
Unit cell lengths	142.360, 142.360, 151.540
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	71.180 - 2.390
R-factor	0.1841
Rwork	0.182
R-free	0.21770
RMSD bond length	0.009
RMSD bond angle	1.164
Data reduction software	xia2
Data scaling software	xia2
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1682)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.770	2.450
High resolution limit [Å]	2.390	2.390
Rmerge	0.137	2.338
Number of reflections	36414
<I/σ(I)>	18.8	1.4
Completeness [%]	99.9	99.6
Redundancy	19.4	18.6
CC(1/2)		0.632

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.1M HEPES pH 7.5, 0.8M Pottassium Phosphate- dibasic, 0.8M Sodium Phosphate monobasic