5LVT
Structure of HU protein from Lactococcus lactis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-03 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.984 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 47.774, 99.002, 168.281 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.030 - 2.100 |
| R-factor | 0.186 |
| Rwork | 0.185 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mul |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.030 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.076 | 0.895 |
| Number of reflections | 22969 | |
| <I/σ(I)> | 11.4 | 1.9 |
| Completeness [%] | 97.3 | 99.5 |
| Redundancy | 5.1 | 5.1 |
| CC(1/2) | 0.998 | 0.593 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 100mM Na acetate, 2M ammonium sulfate |
