5LV4
Crystal structure of mouse PRMT6 in complex with inhibitor LH1236
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97779 |
Spacegroup name | I 41 |
Unit cell lengths | 79.851, 79.851, 119.738 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.080 - 1.660 |
R-factor | 0.16 |
Rwork | 0.159 |
R-free | 0.18820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4c07 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.079 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10pre_2104: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.080 | 1.690 |
High resolution limit [Å] | 1.660 | 1.660 |
Rmerge | 0.142 | 1.508 |
Number of reflections | 44207 | |
<I/σ(I)> | 7.7 | 1 |
Completeness [%] | 99.7 | 94.5 |
Redundancy | 7.2 | 5.5 |
CC(1/2) | 0.996 | 0.474 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | HEPES NaOH pH 8.0 100 mM PEG 6000 10 % MgCl2 200 mM |