5LUX
Homeobox transcription factor CDX1 bound to methylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-03 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9724 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 64.650, 45.555, 116.499 |
| Unit cell angles | 90.00, 93.79, 90.00 |
Refinement procedure
| Resolution | 58.120 - 3.230 |
| R-factor | 0.2467 |
| Rwork | 0.241 |
| R-free | 0.29950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ltx |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.852 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.25) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.120 | 3.490 |
| High resolution limit [Å] | 3.230 | 3.230 |
| Rmerge | 0.174 | 0.533 |
| Number of reflections | 5425 | |
| <I/σ(I)> | 5.3 | 1.91 |
| Completeness [%] | 97.2 | 88.9 |
| Redundancy | 3.4 | 3.2 |
| CC(1/2) | 0.995 | 0.891 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG MME (5000),KCl, MgCl2, 2-Methyl propanol, Tris buffer |
