5LUV
Short LOV protein W619_1 in apo-state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | I 41 |
| Unit cell lengths | 109.538, 109.538, 95.209 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.559 - 2.500 |
| R-factor | 0.2129 |
| Rwork | 0.211 |
| R-free | 0.25280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j3w |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.015 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.600 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.101 | 1.908 |
| Number of reflections | 19498 | |
| <I/σ(I)> | 12.3 | 1.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.7 | 5.9 |
| CC(1/2) | 0.998 | 0.336 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 287 | 1M NH4SO4, 0.1M MES |
