5J3W

Crystal structures reveal signaling states of a short blue light photoreceptor protein PpSB1-LOV (dark state)

Summary for 5J3W

• Entry DOI: 10.2210/pdb5j3w/pdb
• Descriptor: Sensory box protein, FLAVIN MONONUCLEOTIDE (2 entities in total)
• Functional Keywords: signaling protein, lov domain, pas domain
• Biological source: Pseudomonas putida (strain KT2440)
• Total number of polymer chains: 4
• Total formula weight: 76296.70

Authors

Granzin, J.,Batra-Safferling, R. (deposition date: 2016-03-31, release date: 2016-06-22, Last modification date: 2024-01-10)

Primary citation

Rollen, K.,Granzin, J.,Panwalkar, V.,Arinkin, V.,Rani, R.,Hartmann, R.,Krauss, U.,Jaeger, K.E.,Willbold, D.,Batra-Safferling, R.
Signaling States of a Short Blue-Light Photoreceptor Protein PpSB1-LOV Revealed from Crystal Structures and Solution NMR Spectroscopy.
J.Mol.Biol., 428:3721-3736, 2016

PubMed Abstract: Light-Oxygen-Voltage (LOV) domains represent the photo-responsive domains of various blue-light photoreceptor proteins and are widely distributed in plants, algae, fungi, and bacteria. Here, we report the dark-state crystal structure of PpSB1-LOV, a slow-reverting short LOV protein from Pseudomonas putida that is remarkably different from our previously published "fully light-adapted" structure [1]. A direct comparison of the two structures provides insight into the light-activated signaling mechanism. Major structural differences involve a~11Å movement of the C terminus in helix Jα, ~4Å movement of Hβ-Iβ loop, disruption of hydrogen bonds in the dimer interface, and a~29° rotation of chain-B relative to chain-A as compared to the light-state dimer. Both crystal structures and solution NMR data are suggestive of the key roles of a conserved glutamine Q116 and the N-cap region consisting of A'α-Aβ loop and the A'α helix in controlling the light-activated conformational changes. The activation mechanism proposed here for the PpSB1-LOV supports a rotary switch mechanism and provides insights into the signal propagation mechanism in naturally existing and artificial LOV-based, two-component systems and regulators.

PubMed: 27291287
DOI: 10.1016/j.jmb.2016.05.027

Experimental method

X-RAY DIFFRACTION (2.55 Å)