5LUP
Structures of DHBN domain of human BLM helicase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.7 |
| Spacegroup name | P 62 |
| Unit cell lengths | 132.795, 132.795, 64.984 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.470 - 2.032 |
| R-factor | 0.2129 |
| Rwork | 0.210 |
| R-free | 0.26260 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.952 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.470 | |
| High resolution limit [Å] | 2.032 | 2.105 |
| Rmerge | 0.074 | 0.534 |
| Number of reflections | 41409 | |
| <I/σ(I)> | 18.34 | 1.84 |
| Completeness [%] | 98.0 | 87 |
| Redundancy | 8.2 | 3.2 |
| CC(1/2) | 0.999 | 0.767 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | Tris-HCl 0.1M PH8.5 PEG1500 2% Glycerol 16% |
