5LUG

Crystal structure of human Spindlin-2B protein in complex with ART(M3L)QTA(2MR)KS peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-16
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	0.979490
Spacegroup name	P 1 21 1
Unit cell lengths	66.533, 81.225, 93.967
Unit cell angles	90.00, 99.86, 90.00

Refinement procedure

Resolution	19.902 - 1.700
R-factor	0.1956
Rwork	0.194
R-free	0.22110
RMSD bond length	0.007
RMSD bond angle	1.141
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1682)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.126	0.532
Rmeas	0.145	0.623
Rpim	0.071	0.320
Number of reflections	107508
<I/σ(I)>	11.1	2.9
Completeness [%]	99.4	98.3
Redundancy	3.8	3.7
CC(1/2)	0.995	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	25% PEG3350, 0.1M Bis-Tris pH 5.5