5LU6
Heptose isomerase mutant - H64Q
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.610, 84.610, 127.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.310 - 1.670 |
R-factor | 0.19104 |
Rwork | 0.189 |
R-free | 0.22599 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.792 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 63.700 |
High resolution limit [Å] | 1.670 |
Number of reflections | 1322870 |
<I/σ(I)> | 7.4 |
Completeness [%] | 88.6 |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 277 | untagge GmhA 10 mg per ml 0.1 M Na Acetate, pH 4.6, 8% PEG 4000 |