5LTZ
GmhA_mutant Q175E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.550, 84.473, 126.682 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.250 - 1.670 |
R-factor | 0.2095 |
Rwork | 0.207 |
R-free | 0.24470 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.059 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.870 |
High resolution limit [Å] | 1.670 |
Number of reflections | 90441 |
<I/σ(I)> | 11.8 |
Completeness [%] | 98.4 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 277.15 | B. pseudomallei GmhA at 10 mg per mL was mixed with an equal volume of crystallization solution: 0.1 M Na Acetate, pH 4.6, 8% PEG 4000 |