5LTN

Crystal structure of Lymphocytic choriomeningitis mammarenavirus endonuclease complexed with DPBA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2015-07-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978
Spacegroup name	P 41
Unit cell lengths	107.185, 107.185, 53.807
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.593 - 1.880
R-factor	0.1915
Rwork	0.190
R-free	0.21560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ltf
RMSD bond length	0.010
RMSD bond angle	1.101
Data reduction software	XDS
Data scaling software	Aimless (0.5.12)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.593	1.947
High resolution limit [Å]	1.880	1.880
Rmerge	0.094	0.868
Rmeas	0.105	1.150
Number of reflections	241187
<I/σ(I)>	8.54	1.05
Completeness [%]	100.0	100
Redundancy	4.8	2.9
CC(1/2)	0.996	0.416

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0,1M pH 6,2, Isopropanol 3,5%, MgCl2 1mM, DPBA 2mM