5LTN
Crystal structure of Lymphocytic choriomeningitis mammarenavirus endonuclease complexed with DPBA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2015-07-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.978 |
Spacegroup name | P 41 |
Unit cell lengths | 107.185, 107.185, 53.807 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.593 - 1.880 |
R-factor | 0.1915 |
Rwork | 0.190 |
R-free | 0.21560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ltf |
RMSD bond length | 0.010 |
RMSD bond angle | 1.101 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.12) |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.593 | 1.947 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.094 | 0.868 |
Rmeas | 0.105 | 1.150 |
Number of reflections | 241187 | |
<I/σ(I)> | 8.54 | 1.05 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.8 | 2.9 |
CC(1/2) | 0.996 | 0.416 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0,1M pH 6,2, Isopropanol 3,5%, MgCl2 1mM, DPBA 2mM |