5LTF

Crystal structure of Lymphocytic choriomeningitis mammarenavirus endonuclease complexed with catalytic ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2015-03-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9762
Spacegroup name	P 41
Unit cell lengths	108.361, 108.361, 54.106
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.460 - 2.430
R-factor	0.188
Rwork	0.184
R-free	0.23360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jsb
RMSD bond length	0.009
RMSD bond angle	1.082
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.460	2.520
High resolution limit [Å]	2.430	2.430
Rmerge	0.046
Number of reflections	67793
<I/σ(I)>	13.61
Completeness [%]	96.0	96
Redundancy	2.9	2.9
CC(1/2)	0.999	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	sodium citrate 0,1M pH 6,2, Isopropanol 3,5%, MgCl2 1mM