5LRF
Crystal structure of Glycogen Phosphorylase b in complex with KS389
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2015-09-11 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.04 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.560, 128.560, 116.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.140 - 1.750 |
R-factor | 0.16628 |
Rwork | 0.165 |
R-free | 0.18507 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.010 |
RMSD bond angle | 1.430 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.380 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.050 | 0.427 |
Number of reflections | 90698 | |
<I/σ(I)> | 15.1 | 2.9 |
Completeness [%] | 93.2 | 95.5 |
Redundancy | 4.1 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.8 | 289 | 10mM BES buffer |