5LRC
Crystal structure of Glycogen Phosphorylase in complex with KS114
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-2 |
Synchrotron site | MAX II |
Beamline | I911-2 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2015-09-09 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0403 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.410, 128.410, 116.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.640 - 2.000 |
R-factor | 0.16257 |
Rwork | 0.161 |
R-free | 0.19661 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.010 |
RMSD bond angle | 1.311 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.330 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.096 | 0.437 |
Number of reflections | 61240 | |
<I/σ(I)> | 8 | 3 |
Completeness [%] | 94.1 | 96.3 |
Redundancy | 4.2 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.8 | 289 | 10mM Bes buffer |