5LR7
CRYSTAL STRUCTURE OF HSP90 IN COMPLEX WITH SAR567530
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 66.628, 98.223, 90.539 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.940 - 1.860 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yjx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.270 | 1.900 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.272 | |
| Number of reflections | 24806 | |
| <I/σ(I)> | 13.4 | 4.5 |
| Completeness [%] | 97.3 | 64.5 |
| Redundancy | 4.6 | 3.9 |
| CC(1/2) | 0.902 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 1.0 - 1.4M Sodium Citrate |
